Part:BBa_K1357004:Experience

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Applications of BBa_K1357004

Crystal Violet Assay

Mechanism of the assay: Crystal violet binds to polysaccharides of the biofilm matrix. By measuring the binding to adhering biofilm, the amount of biofilm produced can be quantified. A stock solution of crystal violet is incubated with the culture, the supernatant is removed, bound crystal violet is mobilised with an acetic acid solution and the absorption is determined. The absorption values determine the amount of biofilm present in the culture at the time of staining.

The nhaR BioBrick with the constitutive promoter-NC in Figure 1 showed a 33% increase in absorbance compared to the circular pSB1C3. However, we were not able to replicate the results in further trials. This failure to replicate results can be attributed to experimental inconsistencies in later trials.

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